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Isolation and sequence determination of porcine class II DRB alleles amplified by PCR
Author(s) -
Våge D I,
Olsaker I,
Lingaas F,
Lie Ø
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00082.x
Subject(s) - biology , pseudogene , genetics , locus (genetics) , allele , gene , polymerase chain reaction , variants of pcr , microbiology and biotechnology , exon , nucleic acid sequence , genome
Summary The first domain exon of a porcine DRB gene was amplified by the polymerase chain reaction (PCR), and the nucleotide sequence was determined. In a material consisting of 10 unrelated animals, five different alleles were identified, all probably belonging to a single locus designated DRB1. In addition, a non‐expressed locus, designated DRBP, was coamplified with DRB1. This pseudogene, containing a single base deletion, also exhibited some variation, but at a very restricted level compared with DRB1. In pairwise comparisons of DRB1 alleles, the number of amino acid substitutions ranged between 6 and 21 out of 83 positions compared.