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Somatic cell mapping of the bovine prion protein gene and restriction fragment length polymorphism studies in cattle and sheep
Author(s) -
Ryan A M,
Womack J E
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00914.x
Subject(s) - prnp , biology , scrapie , restriction fragment length polymorphism , microbiology and biotechnology , genetics , restriction enzyme , bovine spongiform encephalopathy , gene , virology , genotype , prion protein , medicine , disease , pathology
Summary Brains affected by the progressive neurological disease bovine spongiform encephalopathy (BSE) contain scrapie‐associated fibrils and the protease‐resistant isoform of prion protein. The gene encoding the normal host prion protein (PRNP) has been mapped to human chromosome 20 and mouse chromosome 2 with the hamster cDNA probe pEA974. Using this probe and a panel of bovine‐rodent hybrid somatic cells, we have mapped PRNP to bovine syntenic group U11 (100% concordancy). PRNP restriction fragment length polymorphisms (RFLPs) were detected with five of six enzymes ( Bgl II, Eco RI, Hin dIII, Msp I and Taq I) in sheep, in contrast to one of 16 enzymes ( Hin cII) in cattle. Codominant segregation of the bovine Hin cII RFLP was demonstrated in six backcross pedigrees. While PRNP RFLPs are tightly linked to scrapie incubation period, and consequently susceptibility or resistance to disease in rodents and sheep, the relationship between the PRNP RFLPs and BSE incubation period has not been determined.