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Type I interferon genes in cattle: restriction fragment length polymorphisms, gene numbers and physical organization on bovine chromosome 8
Author(s) -
Ryan A M,
Womack J E
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00912.x
Subject(s) - biology , restriction fragment length polymorphism , locus (genetics) , genetics , genetic linkage , gene , allele , pulsed field gel electrophoresis , loss of heterozygosity , microbiology and biotechnology , genotype
Summary Multiple, superimposed Type I interferon (IFN) restriction fragments were resolved following 72–92 h of horizontal electrophoresis. Restriction fragment length polymorphisms (RFLPs) for α IFN ( IFNA ), β IFN ( IFNB ), ω IFN ( IFNW ) and trophoblast IFN ( IFNT ) genes were identified in Hin dill, Eco RI and Taql digestions from 313 cattle. RFLPs with codominant segregation in cattle pedigrees were considered alleles, and 19 distinct polymorphic Type I IFN loci (5 IFNA , 4 IFNB , 8 IFNW and 2 IFNT ) were identified. Allele frequencies and observed heterozygosity values were calculated for each locus and several loci were considered highly informative for linkage analysis. Bovine IFN gene numbers (10 IFNA , 6 IFNB , 20 IFNW and 6 IFNT ) were estimated from the number of polymorphic loci plus additional monomorphic hybridizing bands present in Eco RI and Hindlll digestions. Physical linkage of the Type I IFN gene families on bovine chromosome 8 was demonstrated by pulsed field gel electrophoresis (PFGE). Hybridization of two or more IFN probes to similarly sized PFGE fragments suggested the tentative gene family order: IFNA/IFNW‐IFNT‐IFNB. These studies provide a basis for the development of more detailed genetic and physical maps of the bovine Type I IFNs.