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Restriction fragment length polymorphism of DQB and DRB class II genes of the ovine major histocompatibility complex
Author(s) -
Grain F.,
Grain F.,
Nain MC,
Asso J.,
Labonne MP,
Gebuhrer L.,
Betuel H.,
Lantier F.,
Lechopier P.,
Maddox J.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00344.x
Subject(s) - restriction fragment length polymorphism , taqi , ecori , biology , hindiii , restriction enzyme , southern blot , microbiology and biotechnology , genetics , bamhi , exon , restriction fragment , immunogenetics , gene , histocompatibility , human leukocyte antigen , antigen , genotype
Summary The ovine major histocompatibility complex (MhcOvar) class II region was investigated by Southern blot hybridizations using ovine probes specific for the second exons of Ovar‐DRB and Ovar‐DQB genes. Multiple bands were revealed when genomic DNA was digested with each of five restriction enzymes ( Bam HI, Eco RI, Hin dIII, Pvu II and Taq I), and successively hybridized with the two radiolabeled ovine probes. Restriction fragment length polymorphisms (RFLPs) were analysed in 89 sheep originating from six inbred families and the inheritance of the fragment patterns was determined. Forty‐one fragments were recorded with the DQB probe; 32 were detected with the DRB probe. They constituted 9 DQB and 10 DRB allelic patterns. Twelve DQB‐DRB haplotypes were resolved in this study.