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Designation by restriction fragment length polymorphism of major histocompatibility complex class IV haplotypes in meat‐type chickens
Author(s) -
Landesman E.,
Uni Z.,
Heller E. D.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00339.x
Subject(s) - biology , restriction fragment length polymorphism , haplotype , restriction enzyme , genetics , major histocompatibility complex , restriction fragment , restriction site , bglii , terminal restriction fragment length polymorphism , population , polymorphism (computer science) , microbiology and biotechnology , genotype , gene , hindiii , demography , sociology
Summary Major histocompatiblity complex (MHC) class IV haplotypes were identified in a population of meat‐type chickens by restriction fragment length polymorphism (RFLP) analysis. Fourteen different haplotypes were designated on the basis of restriction patterns obtained from Southern blots of Pvu II‐ or Bgl II‐digested DNA, hybridized with the MHC class IV cDNA probe bg32.1. Digestion with each restriction enzyme yielded the same level of polymorphism among individuals. For each haplotype, 4–10 restriction fragments ranging from 0–8 to 8 kb were observed. Such a designation of meat‐type chicken MHC class IV haplotypes enables a rapid recognition of previously defined haplotypes, is readily adjustable to additional, newly found restriction patterns and could prove useful in practical breeding programmes.