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The duplicated gene copy of the ovine growth hormone gene contains a Pvu II polymorphism in the second intron
Author(s) -
Sise J. A.,
Penty J. M.,
Montgomery G. W.,
Gootwine E.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00321.x
Subject(s) - biology , restriction fragment length polymorphism , intron , genetics , locus (genetics) , gene , allele , microbiology and biotechnology , polymerase chain reaction , growth hormone , polymorphism (computer science) , hormone , biochemistry
Summary Pvu II restriction fragment length polymorphism (RFLP) was found at the growth hormone locus in sheep carrying the GH2 allele where the gene is duplicated. By restriction analysis and using the polymerase chain reactibn we demonstrated that this RFLP is due to a mutation at the Pvu II site located in the second intron of the 3′ copy of the GH2 allele.