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An additional locus ( PI4 ) in the protease inhibitor ( PI ) gene cluster of pigs
Author(s) -
Ĉizova D.,
Gábriŝová E.,
Stratil A.,
Hojný J.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00320.x
Subject(s) - biology , locus (genetics) , protease , gene cluster , gene , microbiology and biotechnology , genetics , biochemistry , enzyme
Summary A further α‐protease inhibitor system, PI4, was detected in porcine sera using either 2D agarose gel, pH 5.0‐PAGE, pH 9.0, or ID PAGE followed by immunoblotting with rabbit anti‐porcine PI2 or PI3 antisera. PI4 inhibited chymotrypsin, but not trypsin. Seven allelic variants of PI4 were described. By haplotyping of a‐protease inhibitor systems in 52 complete families it was shown that PI4 locus belongs to the PI gene cluster. The probable order of the PI loci was: PI 1, PO1A, PI2, PI4, PI3.