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Preparation of chromosome‐specific paints and complete assignment of chromosomes in the pig flow karyotype
Author(s) -
Langford C. F.,
Miller N. G. A.,
Tucker E. M.,
Telenius H.,
Thomsen P. D.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00309.x
Subject(s) - karyotype , biology , metaphase , chromosome , fluorescence in situ hybridization , genetics , microbiology and biotechnology , fish <actinopterygii> , chromomycin a3 , gene , fishery
Summary Sorted chromosomes from each of the 20 clusters of the male porcine bivariate flow karyotype were amplified and biotinylated using DOP‐PCR. The chromosomes comprising each cluster were identified by fluorescence in situ hybridization (FISH) of the 20 probes to R‐banded male pig metaphase spreads. A standard flow karyotype for the pig is presented.