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Double‐strand DNA conformation polymorphisms as a source of highly polymorphic genetic markers
Author(s) -
Kirkpatrick B. W.,
Huff B. M.,
CasasCarrillo E.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00280.x
Subject(s) - biology , restriction fragment length polymorphism , genetics , haplotype , dna , intron , allele , exon , genetic marker , cleaved amplified polymorphic sequence , gene , polymorphism (computer science) , restriction fragment , microbiology and biotechnology , restriction site , base pair , dna sequencing , polymerase chain reaction , restriction enzyme
Summary The molecular basis for several apparent restriction fragment length polymorphisms of the porcine growth hormone gene was examined through DNA sequence analysis. Electrophoretic and sequence analysis suggest polymorphisms result from 1–3 base substitutions that affect double‐strand DNA conformation and electrophoretic mobility. Two allelic forms of the porcine growth hormone 5'flank and four allelic forms of the second exon/intron region were identified. A marker system was developed which combined conformation polymorphisms with Hae II and Dde I RFLPs. Using this system, nine haplotypes were observed in samples from three US swine breeds. The data presented suggest that double‐strand DNA conformation can be exploited in base substitution detection and development of highly polymorphic genetic marker systems.