Premium
A polymorphism in the bovine gamma‐S‐crystallin gene revealed by allele‐specific amplification
Author(s) -
Kemp S. J.,
Teale A. J.,
Maillard J. C.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00253.x
Subject(s) - biology , microbiology and biotechnology , polymerase chain reaction , agarose gel electrophoresis , genomic dna , allele , genetics , variants of pcr , gene , single nucleotide polymorphism , polymorphism (computer science) , genotype
Summary A polymorphism was detected in the 3′ untranslated region of the bovine gamma‐S‐crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele‐specific amplification. The two allele‐specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism.