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Isolation and mapping of polymorphic microsatellites in cattle
Author(s) -
Steffen P.,
Eggen A.,
Stranzinger G.,
Fries R.,
Dietz A. B.,
Womack J. E.
Publication year - 1993
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1993.tb00252.x
Subject(s) - biology , microsatellite , genetics , isolation (microbiology) , computational biology , evolutionary biology , gene , allele , bioinformatics
Summary A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC‐dA) n ‐(dG‐dT) n oligonucleotide probe for the repeated nucleotide motif (CA) n . Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR‐analysis of a panel of somatic hybrid lines.