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Detection of bovine α s1 ‐casein genomic variants using the allele‐specific polymerase chain reaction
Author(s) -
DAVID V. A.,
DEUTCH A. H.
Publication year - 1992
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1992.tb02161.x
Subject(s) - polymerase chain reaction , genotype , biology , casein , allele , isoelectric focusing , microbiology and biotechnology , typing , genetics , gene , biochemistry , enzyme
Summary. A method was developed to distinguish between genotypic variants B and C of bovine α s1 ‐casein, using the allele‐specific polymerase chain reaction (ASPCR). The α s1 ‐casein genotype determined for 17 Jersey cows by the ASPCR method was confirmed by typing the α s1 ‐casein milk proteins on isoelectric focusing gels. Using the ASPCR method described, rapid analysis of the α s1 ‐casein genotype of bulls is now possible. In addition, K‐casein genotypes can be determined from the same PCR reaction.