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Molecular cloning and sequence analysis of a chicken ornithine decarboxylase cDNA
Author(s) -
JOHNSON R.,
BULFIELD G.
Publication year - 1992
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1992.tb02158.x
Subject(s) - complementary dna , biology , microbiology and biotechnology , peptide sequence , cdna library , cloning (programming) , molecular cloning , sequence analysis , coding region , ornithine decarboxylase antizyme , clone (java method) , ornithine decarboxylase , genetics , gene , biochemistry , enzyme , computer science , programming language
Summary. A chicken embryo cDNA library was screened with a mouse probe for ornithine decarboxylase (ODC) and 14 positively hybridizing clones isolated. The longest of these (1.7 kb) was sub‐cloned and sequenced. It is estimated that the clone comprises approximately 98% of the coding region for chicken ODC. The DNA sequence shows 78% identity with the human ODC cDNA sequence and the deduced amino acid sequence is almost 90% homologous to mouse and human. Both the peptide and cDNA sequences show interesting potential regulatory features which are discussed here.