Premium
Restriction fragment length polymorphism analysis of major histocompatibility complex class IV (B‐G) genotypes in meat‐type chickens
Author(s) -
UNI Z.,
HILLEL J.,
WAIMAN R.,
CAHANER A.,
HELLER D. E.
Publication year - 1992
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1992.tb00162.x
Subject(s) - biology , restriction fragment length polymorphism , genotype , restriction enzyme , genotyping , genetics , polymorphism (computer science) , terminal restriction fragment length polymorphism , major histocompatibility complex , restriction fragment , cleaved amplified polymorphic sequence , microbiology and biotechnology , gene
Summary. Restriction fragment length polymorphism (RFLP) was used as a molecular genotyping approach to characterize differences in major histocompatibility complex class IV genes in meat‐type chickens. A high level of polymorphism was observed following digestion with each of the two restriction endonucleases Pvu II and Bgl II. Examination of DNA from 54 chickens revealed 23 polymorphic fragments. Application of RFLP techniques in the analysis of family groups should make possible the determination of B‐G genotypes in the meat type chickens.