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Genetic analysis of fingerprints in Mérinos d'Arles X Booroola Merino crossbred sheep
Author(s) -
LANNELUC I.,
HOSPITAL F.,
CHEVALET C.,
ELSEN J. M.,
GELLIN J.
Publication year - 1992
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1992.tb00156.x
Subject(s) - minisatellite , biology , genetics , dna profiling , crossbreed , genetic marker , dna , microsatellite , genetic linkage , gene , allele
Summary. The M13.13 minisatellite probe, consisting of a polymer of the M13 VNTR consensus sequence, cross‐hybridized to ovine DNA and allowed detection of several polymorphic loci. Individual specific patterns were obtained in sheep using this probe. Pedigree analysis showed that individuals were heterozygous for most of the DNA fragments detected (88%). By studying the segregation of male's variable DNA fragments, a minimum of 10 loci were defined. The ovine DNA ‘fingerprint’ obtained with M13.13 is polymorphic enough to be used efficiently in animal identification, paternity testing, and possibly as a source of genetic markers for linkage analysis.