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Multiple restriction fragment length polymorphisms in the porcine calcium release channel gene (CRC): assignment to the halothane (HAL) linkage group
Author(s) -
MARIANI P.,
JOHANSSON M.,
ELLEGREN H.,
HARBITZ I.,
JUNEJA R. K.,
ANDERSSON L.
Publication year - 1992
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1992.tb00138.x
Subject(s) - restriction fragment length polymorphism , biology , genetics , locus (genetics) , genetic linkage , microbiology and biotechnology , allele , gene , gene mapping , genotype , chromosome
Summary. Two cDNA probes for the porcine calcium release channel gene ( CRC ) were used in restriction fragment length polymorphism (RFLP) analysis in an attempt to develop genetic markers linked to the malignant hyperthermia (stress susceptibility) gene ( HAL ). Three Taq I RFLPs, denoted CRC1‐CRC3, each composed of two alleles, were detected. RFLPs were also detected with Msp I and Pvu II, but the Msp I RFLP correlated completely with CRC3 in this material and the Pvu II RFLP could not be scored reliably due to a minute size difference between the two allelic fragments. The autosomal codominant inheritance of these RFLP loci was confirmed by family analyses. Significant evidence for genetic linkage between the CRC1/CRC3 loci and the A1BG locus in the HAL linkage group confirmed a previous assignment of the CRC gene to chromosome 6 in the pig.