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Organization and polymorphism of bovine major histocompatibility complex class II genes as revealed by genomic hybridizations with bovine probes
Author(s) -
SIGURDARDÓTTIR S.,
MARIANI P.,
GROENEN † M.A.M.,
VAN J.,
POEL DER,
ANDERSSON L.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00718.x
Subject(s) - biology , restriction fragment length polymorphism , genetics , genomic dna , hybridization probe , gene , bovine genome , molecular probe , restriction enzyme , restriction fragment , microbiology and biotechnology , genome , polymerase chain reaction
Summary. Previous studies on restriction fragment length polymorphism of bovine major histocompatibility complex class II genes have primarily been based on the use of human probes. In the present study bovine probes for DQA, DQB, DRB and DYA were used for RFLP analysis of cattle genomic DNA digested with Pvu II and Taq I. There was an excellent agreement between the RFLP results obtained with homologous and heterologous probes. Although a few ‘new’ restriction fragments were revealed with the bovine probes there was no discrepancy with regard to the classification of allelic types with the two types of probes. The major advantages of using bovine probes were a better hybridization signal and reduced cross‐hybridization between loci. Hybridization experiments with DQA probes for the first domain exon from two different genomic clones revealed the presence of two distinct types of bovine DQA genes. Surprisingly, these probes did not cross‐hybridize at high stringency, indicating that the two genes are quite divergent. Hybridization with a recently described genomic clone for a novel bovine α‐chain gene confirmed that it corresponds to the DYA gene which had previously been identified by cross‐hybridization to a human DQA probe.