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Nucleotide sequence and Northern analysis of a bovine major histocompatibility class II DRß‐like cDNA
Author(s) -
BURKE M. G.,
STONE R. T.,
MUGGLICOCKETT N. E.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00688.x
Subject(s) - biology , complementary dna , major histocompatibility complex , genetics , sequence (biology) , nucleic acid sequence , sequence analysis , computational biology , gene , microbiology and biotechnology
Summary. A1.2‐kb bovine DRß‐like cDNA clone (BoLA‐DRB3) was isolated from a peripheral blood lymphocyte cDNA library utilizing a human DRß cDNA as a probe. BoLA‐DRB3 was found to have a high degree of nucleotide sequence similarity (96.8%) with a previously sequenced bovine DRß‐like gene (Al). It is believed that BoLA‐DRB3 and A1 represent distinct alleles of one of the three bovine DRß‐like loci. Sequence comparison of BoLA‐DRB3 with genes representing the other two bovine DRß‐like loci. resulted in moderate degrees of sequence similarities (83.1% and 86.3%, respectively). Comparison of the relative abundance of RNA transcripts of the three bovine DRß‐like loci by Northern analysis of lymphocyte RNA indicated that BoLA‐DRB3 is the most actively transcribed of the three bovine DRß‐like genes. Based on these results we suggest that of the three DRß‐like loci thus far identified in the bovine, only one is actively transcribed.