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Class II major histocompatibility complex genes of the sheep
Author(s) -
DEVERSON E. V.,
WRIGHT H.,
WATSON S.,
BALLINGALL K.,
HUSKISSON N.,
DIAMOND A. G.,
HOWARD J. C.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00671.x
Subject(s) - cosmid , biology , gene , major histocompatibility complex , genetics , human leukocyte antigen , gene mapping , histocompatibility , microbiology and biotechnology , antigen , chromosome
Summary. The class II genes of the sheep major histocompatibility complex (MHC) have been cloned from two unrelated heterozygous sheep into cosmid vectors. By restriction mapping and hybridization with a number of class II probes of human and mouse origin, the cloned genetic material has been assigned to seven distinct α genes, 10 distinct ß genes and 14ß‐related sequences. It was difficult to identify homologues of specific HLA class II genes because of a tendency for the ovine genes to cross‐hybridize between HLA probes representing different loci. Such cross‐hybridization was especially marked among the ß genes. While DQ and DR homologues have been tentatively identified by several criteria, no genes corresponding to DP have been identified. Cosmids containing class II α and ß genes have been transfected into mouse LTK‐ cells, and surface expression of a sheep class II molecule has been obtained.