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Mouse monoclonal antibodies to bovine blood group antigens: additional results
Author(s) -
METENIER L.,
NOCART M.,
ALAUX M. T.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00657.x
Subject(s) - biology , antigen , antibody , polyclonal antibodies , monoclonal antibody , epitope , virology , spleen , microbiology and biotechnology , monoclonal , immunology
Summary. Seven fusions of mouse myeloma cells with spleen cells from mice immunized with bovine red cells yielded 61 clones producing discriminant antibodies out of a total of 651 secreting clones. Although antigenic factors of all known bovine blood group systems were present on the donors' cells, the antibodies identified reacted with antigenic factors from only five systems, A, B, F, S and Z. The antibody specificities produced by more than two clones were anti‐A 1 or ‐A 2 (21 clones), ‐S (9),‐Z (6), ‐G‘ (3) and ‐V 1 (3). The absence of clones secreting antibodies to antigens of the other systems, especially the complex C system, remains unexplained. The properties of the antibodies reacting with antigens of the S system (anti‐SU“, anti‐SUU’) and of the B system (O‐like antibodies) are in accordance with previous interpretations of polyclonal sera and with present knowledge of the genetic map of the B system.