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Genetic mapping by single sperm typing
Author(s) -
ARNHEIM N.,
LI H.,
CUI X.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00652.x
Subject(s) - biology , typing , genetics , sperm , computational biology
Summary. The polymerase chain reaction makes it possible to analyse DNA sequences in a single cell and has led to a new approach for constructing genetic maps. We describe a procedure called ‘sperm typing’ which can accurately classify individual meiotic products as recombinant or non‐recombinant. This permits the linkage relationships among DNA polymorphisms to be determined without pedigree analysis.