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Differential expression of I α‐ and II α‐globin genes in Norwegian dairy goats
Author(s) -
BERGERSEN O.,
FOSSE V. M.,
NESSE L. L.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00648.x
Subject(s) - biology , globin , microbiology and biotechnology , gene , gene duplication , restriction enzyme , phenotype , polyacrylamide gel electrophoresis , genetics , enzyme , biochemistry
Summary. Fast protein liquid chromatography (FPLC) and polyacrylamide gel electrophoresis were used to determine the ratio between I α‐and II α‐globin chains in Norwegian dairy goats. Three different phenotypes, designated normal (N) with I α‐ to II α‐globin ratio 3:1, reversed (R) with ratio 1:2 and double‐reversed (RR) with no I α‐globin, were described. Family studies indicated that the R animals were heterozygous, and the RR animal homozygous, for a haplotype without a functional I α‐globin product. Southern blot analysis of goat DNAs digested with six different restriction enzymes showed that the different ratios of α chain expression could not be due to a deletion of the I α‐gene and/or duplication or triplication of the II α‐globin genes. The homozygous reversed animal with no detectable I α‐globin had a mild anaemia.