Premium
DNA polymorphism at the casein loci in sheep
Author(s) -
GREGORIO P. DI,
RANDO A.,
PIERAGOSTINI E.,
MASINA P.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00643.x
Subject(s) - biology , restriction fragment length polymorphism , genetics , loss of heterozygosity , restriction enzyme , complementary dna , gene , polymorphism (computer science) , dna , genetic variation , microbiology and biotechnology , genotype , allele
Summary. By using seven endonucleases and four bovine cDNA probes specific for α s1‐, α s2‐, ß‐, and K‐casein genes, nine restriction fragment length polymorphisms (RFLPs) have been found in the sheep orthologous DNA regions. In contrast to the low level of variation observed at the protein level, these DNA polymorphisms determine a high level of heterozygosity and, therefore, represent useful tools for genetic analyses since they can also be obtained without the need for gene expression. In fact, informative matings suggest that in sheep, as in cattle, the four loci are linked.