Premium
Restriction fragment length polymorphism of ovine casein genes: close linkage between the α s1‐, α s2‐, ß‐ and k‐casein loci
Author(s) -
LEVEZIEL H.,
METENIER L.,
GUERIN G.,
CULLEN P.,
PROVOT C.,
BERTAUD M.,
MERCIER J. C.
Publication year - 1991
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1991.tb00641.x
Subject(s) - restriction fragment length polymorphism , biology , restriction enzyme , hindiii , genetics , locus (genetics) , restriction fragment , genetic linkage , microbiology and biotechnology , gene , restriction map , casein , southern blot , genomic dna , allele , plasmid , genotype , biochemistry
Summary. Restriction fragment length polymorphism (RFLP). of ovine casein genes was investigated. Genomic DNA from 56 rams was digested with 10 restriction endonucleases and Southern blots probed with the four ovine casein cDNAs (α s1‐, ß‐, α s2‐ and k‐Cn). Five enzymes, namely, Bgl I, Pvu II, Rsa I, Taq I and Hind III revealed nine different RFLPs. The inheritance of six of these polymorphisms was studied by segregation analysis of gametes in nine rams' families, and each of them could be related to the existence of alleles at the relevant casein locus. A close linkage between the four ovine casein genes was demonstrated since no recombination within the four pairs of loci examined, α s1 ‐ß‐Cn, α s1 ‐k‐Cn, ß‐k‐Cn and α s2 ‐k‐Cn, was observed in the progeny of double heterozygous rams. The casein genes are thus clustered in the ovine species as in the case of other mammals.