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Polymorphism of pig serum α‐protease inhibitor‐3 (PI3) and assignment of the locus to the Pi1, Po1A, Po1B, Pi2, Igh linkage group
Author(s) -
STRATIL A.,
CIZOVÁ D.,
HOJNÝ J.,
HRADECKÝ J.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb03236.x
Subject(s) - locus (genetics) , biology , microbiology and biotechnology , allele , protease , genetics , typing , enzyme , gene , biochemistry
Summary Polymorphism of an α‐protease inhibitor, PI3, in pig serum samples was detected using 2D agarose gel (pH 5.4) — polyacrylamide gel (pH 9.0) electrophoresis. Evidence was obtained that the five variants observed (A, B1, B2, C and D) are under genetic control by codominant alleles ( Pi3 A , Pi3 B1 , Pi3 B2 , Pi3 C and Pi3 D ) at one autosomal locus. Variants A, B1, B2 and C inhibited chymotrypsin; there was no appreciable inhibition of trypsin and papain. Variant D did not inhibit chymotrypsin, and therefore its classification as a PI3 variant was put in question. PI3 typing was not possible in about 50% of the studied pigs since in those cases the PI3 variants were either too weak or absent. On the basis of backcross matings and haplotyping in complete families for protease inhibitor loci Pi1, Po1A, Pi2 and Pi3 it was proved that the Pi3 locus belongs to the protease inhibitor gene cluster, and the position of the locus in the linkage group was proposed as being Pi1‐Po1A‐(PolB)‐Pi3‐Pi2‐(Igh1, Igh2, Igh3, Igh4) .