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Monoclonal antibodies to the bovine immunoglobulin G2a allotypes A1 and A2
Author(s) -
ESTES D. M.,
TEMPLETON J. W.,
ADAMS L. G.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb03223.x
Subject(s) - epitope , allotype , monoclonal antibody , biology , antibody , epitope mapping , linear epitope , microbiology and biotechnology , virology , immunology
Summary Production of murine monoclonal antibodies (Mabs) to bovine IgG2a‐A1 and A2 allotypes resulted in three Mabs being selected as anti‐IgG2a‐A1 and A2 reagents. Two Mabs recognize the A1 allotype and 1 recognizes the A2 allotype. Initial epitope mapping with the Mabs indicates that one of the A1 epitopes resides in the hinge region and the other epitope resides more toward the C‐terminus of the immunoglobulin. The A2 epitope recognized by the A2 Mab does not appear to reside in the hinge region of the immunoglobulin but apparently resides more C‐terminal.