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Identification of goat allotypic determinants and generation of goat‐mouse hybridomas secreting goat IgG2
Author(s) -
CAPPARELLI R.,
SORBO G.,
IANNELLI D.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb03222.x
Subject(s) - biology , monoclonal antibody , gene , macroglobulin , antibody , antiserum , microbiology and biotechnology , immunology , genetics
Summary Five allotypic determinants controlled by independent genes have been identified in goat. Of these determinants, four have been detected with alloimmune antisera and one with monoclonal antibodies. The specificities A1, C1 and D1 are lipoproteins; B1 is possibly an α2 macroglobulin and E1 an IgG2. The specificity B1 is not expressed until the age of 3–4 months. The gene controlling the specificity E1 is present at about the same frequency (0·38‐0·41) in goat, sheep, cattle and water buffalo. Stable hybridomas secreting goat IgG2 have been obtained by the fusion of goat peripheral lymphocytes with mouse myeloma cells.