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A bovine monoclonal antibody detecting a class I BoLA antigen
Author(s) -
KEMP S. J.,
TUCKER E. M.,
TEALE A. J.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb03220.x
Subject(s) - biology , monoclonal antibody , epitope , major histocompatibility complex , antigen , antibody , microbiology and biotechnology , peripheral blood mononuclear cell , mhc class i , immunoprecipitation , population , virology , lymph node , immunology , genetics , in vitro , demography , sociology
Summary The bovine IgG 1 monoclonal antibody (mAb) ILA70 was made by immunizing a calf with peripheral blood mononuclear cells (PBM) from a BoLA‐w10 homozygous heifer and subsequently fusing lymphocytes from the local lymph‐node with the heterohybridoma 53B3. Family and population studies, antibody binding inhibition and immunoprecipitation of the target antigen all indicate that ILA70 detects a polymorphic epitope on a bovine class I major histocompatibility complex (MHC) molecule. The antibody is complement fixing and so may be used in a standard cytotoxicity assay. Ascitic fluid with antibody activity many times greater than that of the tissue‐culture supernatant has been prepared in nude mice. The antibody‐producing heterohybridoma has been subcloned three times and appears to be stable. Such heterohybridomas may prove to be a valuable source of particularly discriminating and informative mAbs for the serological analysis of the products of the bovine MHC.