Premium
Restriction fragment length polymorphism analysis of the κ‐casein locus in cattle
Author(s) -
DAMIANI G.,
FERRETTI L.,
ROGI G.,
SGARAMELLA V.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb03214.x
Subject(s) - biology , restriction fragment length polymorphism , terminal restriction fragment length polymorphism , genetics , locus (genetics) , amplified fragment length polymorphism , restriction fragment , polymorphism (computer science) , casein , allele , genotype , dna , gene , food science , population , demography , sociology , genetic diversity
Summary The two common genetic variants (A and B) of bovine κ‐casein originate from two point mutations in the codons for the aminoacids in position 136 and 148. These mutations give rise to polymorphic sites for the restriction endonucleases Hin dII, Alu I, Hin fI, Mbo II and Taq I. We have examined DNAs of several Italian Friesian cows and bulls of known and unknown genotype by Southern analyses using κ‐casein cDNA probes. Restriction fragment length polymorphisms (RFLPs) specific for the A and B alleles were identified for each of the above enzymes, except for Alu I, which has a non‐polymorphic site 12bp away from the polymorphic one. We have also found two new polymorphic sites for Mbo II and Taq I in the non‐coding regions. These sites differentiate the A allele into two new variants, named A 1 and A 2 . The RFLP analysis permits the characterization of κ‐casein alleles even in the absence of their expression. This should facilitate selective breeding programmes aimed at increasing the frequency of the κ‐casein B allele whose product improves the cheesemaking properties of milk.