Premium
Partial nucleotide sequence of a novel bovine major histocompatibility complex class II β‐chain gene, BoLA‐DIB
Author(s) -
STONE R. T.,
MUGGLICOCKETT N. E.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb01980.x
Subject(s) - biology , exon , microbiology and biotechnology , gene , genetics , intron , nucleic acid sequence , complementary dna , genomic dna , peptide sequence , southern blot , bovine genome , genome
Summary. A bovine genomic clone that hybridized to HLA‐DQβ cDNA was isolated and fragments containing the β 1 , β 2 and transmembrane (TM) exons subcloned. The nucleotide sequences of the exons and flanking intron regions were determined. Comparisons of these exon nucleotide sequences and derived amino acid sequences to human class II β ‐chain sequences showed that this gene is only 77% identical to HLA‐DQ β and about 75% identical to bovine DQβ ‐like genes. The exon sequences were more divergent from other class II β ‐chain genes. However, structural features such as conserved cysteines and regions of amino acids strongly suggest this to be a class II β ‐chain gene. When exon‐containing fragments were used as hybridization probes on Southern blots of bovine genomic DNA digested with Eco RI or Pvu II, each exon hybridized to a single band. Based on these results we have referred to this gene as a novel bovine class II β ‐chain gene, BoLA‐DIB.