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Restriction fragment length polymorphism identification of goat α s1 ‐casein alleles: a potential tool in selection of individuals carrying alleles associated with a high level protein synthesis
Author(s) -
LEROUX C.,
MARTIN P.,
MAHÉ M.F.,
LEVÉZIEL H.,
MERCIER J.C.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb01979.x
Subject(s) - biology , allele , genetics , genotype , restriction fragment length polymorphism , locus (genetics) , restriction enzyme , complementary dna , microbiology and biotechnology , mendelian inheritance , casein , gene , biochemistry
Summary. The extensive polymorphism of caprine α s1 ‐casein, which is controlled by at least seven autosomal alleles segregating in a Mendelian fashion, was investigated by RFLP analysis. Genomic DNA from 77 lactating goats, whose genotypes had been previously determined by electrophoretic analysis of milk proteins, was digested with 11 restriction endonucleases and Southern blots were probed with a radiolabeled ovine α s1 ‐casein cDNA. Three enzymes, Pst I, Taq I and Rsa I, allowed the unambiguous identification of known alleles α s1 ‐Cn D , E and F and of the allelic pairs [α s1 ‐Cn B and F ] and [α s1 ‐Cn B and C ]. Evidence for a second null allele, termed α s1 ‐Cn D , and for an additional allele, designated α s1 ‐Cn F , was provided, which leads to the identification of nine alleles at the α s1 ‐CnO locus, in this species. Although only 15 out of the 45 expected genotypes could be fully ascertained, this procedure allows the identification at birth of animals carrying the α s1 ‐Cn A , B or c alleles associated with a high α s1 ‐ and whole‐casein content.