Premium
Two‐dimensional electrophoresis of the plasma proteins of alpacas and llamas: genetic polymorphism of α 1 B‐glycoprotein and three other proteins
Author(s) -
JUNEJA R. K.,
PENEDO M. C.T.,
LARSSON H. E. B.,
GAHNE B.,
BOWLING A. T.
Publication year - 1989
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1989.tb00895.x
Subject(s) - biology , polymorphism (computer science) , blood proteins , allele , glycoprotein , microbiology and biotechnology , antiserum , gel electrophoresis , polyacrylamide gel electrophoresis , electrophoresis , agarose gel electrophoresis , gel electrophoresis of proteins , agarose , genetics , biochemistry , antibody , gene , enzyme
Summary. Plasma samples of alpacas and llamas were analysed by a simple method of two‐dimensional (2‐D) agarose gel (pH 8.6)‐horizontal polyacrylamide gel (pH 9.0) electrophoresis, followed by general protein staining of gels. Genetic polymorphism in both species is described for α 1 B‐glycoprotein (α 1 B) and three other unidentified proteins designated prealbumin (Pr), postalbumin 1 and 2 (Pal and Pa2). α 1 B was identified by cross‐reactivity with antisera for human and pig α 1 B. Altogether, two alleles of Pr, two of Pa1, five of α 1 B and three of Pa2 are described. Most of the alleles were present in alpacas and llamas. Alpacas showed a high degree of polymorphism at all four loci. Llamas showed considerable polymorphism at only the Pa1 and Pa2 loci. The theoretical probability of exclusion (P e ) of an incorrectly assigned parent was estimated to be about 80% in each species by typing for the six polymorphic plasma proteins reported so far in these species. The given method of 2‐D electrophoresis revealed no fixed differences in protein mobilities that discriminate between llamas and alpacas.