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Additional studies of sheep haemopexin: genetic control, frequencies and postnatal development *
Author(s) -
STRATIL A.,
BOBÁK P.,
MARGETIN M.,
GLASNÁK V.
Publication year - 1989
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1989.tb00856.x
Subject(s) - biology , immunoelectrophoresis , allele , endocrinology , medicine , allele frequency , biochemistry , genetics , gene , antigen
Summary. This study presents evidence that sheep haemopexin phenotypes are genetically controlled by three alleles, Hpx A , Hpx B1 and Hpx B2 , of a single autosomal locus. Frequencies of two alleles, Hpx A and Hpx B (Hpx B encompasses two isoalleles, Hpx B1 and Hpx B2 ), were studied in eight sheep breeds in Czechoslovakia. The frequency of the HpxA allele was highest (ranging from 0.81 in Merino to 1.0 in East Friesian sheep). Qualitative and quantitative changes in haemopexin during postnatal development were studied by starch gel electrophoresis and rocket immunoelectrophoresis respectively. In electrophoresis, 1‐ or 2‐day‐old lambs had two very weak zones corresponding in mobility to two slower zones of adult animals. Later, the third more anodic zone appeared and gradually increased in intensity. In 1‐month‐old lambs the patterns were practically identical with those of adult animals. Using rocket immunoelectrophoresis, the level of haemopexin shortly after birth was practically zero. It rose sharply till the sixth day of life; then the level continued to rise slowly till about 1 month of age. The mean haemopexin level in adult sheep was 64.5 ± 18.26 (SD) mg/100ml serum, ranging from 30.5 to 116.5 mg/100ml.