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Absence of BoLA class I antigens on bovine spermatozoa
Author(s) -
MATOUŠEK J.,
SRŠEŇ V.,
SIMON M.,
HOŘíN P.,
LOUDA F.
Publication year - 1989
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1989.tb00843.x
Subject(s) - biology , antigen , sperm , polyclonal antibodies , immunology , artificial insemination , semen , andrology , genetics , pregnancy , medicine
Summary. Forty AI bulls were tested for BoLA class I antigens by means of eight specific polyclonal reagents. By means of immobilization and sperm penetration tests these antigens were not detected on sperm cells. Isoimmunization studies with the use of sperm as antigenic stimuli and insemination of frozen spermatozoa diluted in specific reagents did not prove the presence of BoLA class I antigens on bovine spermatozoa. The cytotoxic tests used in this investigation were not reliable.