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A monoclonal antibody identifying a T‐cell marker in the horse
Author(s) -
CRUMP A. L.,
DAVIS W.,
ANTCZAK D. F.
Publication year - 1988
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1988.tb00826.x
Subject(s) - monoclonal antibody , biology , horse , immunoperoxidase , microbiology and biotechnology , immunofluorescence , flow cytometry , spleen , antibody , antigen , pathology , immunology , medicine , paleontology
Summary. A cell surface molecule of equine T lymphocytes was identified and characterized using a mouse monoclonal antibody, HT23A. The molecule was detected on all T cells but not on other cells in peripheral blood, with the possible exception of a small subpopulation (about 5%) of B cells, as assessed by indirect immunofluorescence and flow cytometry. HT23A labelled T cell areas of horse lymph nodes and spleen when used in an indirect immunoperoxidase assay on frozen sections. Macrophages and neutrophils were not labelled by the antibody nor were frozen sections of horse liver, kidney, or brain. HT23A precipitated a molecule of approximately 69kDa from 125 Iodine labelled horse lymphocytes.