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Genetic polymorphism of plasma α 1 B‐glycoprotein and transferrin in arctic and silver foxes
Author(s) -
JUNEJA R. K.,
NIINI T.,
LOHI O.,
LARSEN B.,
GAHNE B.
Publication year - 1988
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1988.tb00812.x
Subject(s) - transferrin , biology , arctic fox , silver stain , arctic , allele , microbiology and biotechnology , hybrid , glycoprotein , genetics , polyacrylamide gel electrophoresis , zoology , gene , biochemistry , ecology , botany , lagopus , enzyme
Summary. Plasma samples of 235 foxes from 38 complete families (14 of arctic foxes, 21 of silver foxes and 3 with arctic X silver fox hybrid offspring) were analysed by one‐dimensional horizontal polyacrylamide gel electrophoresis (PAGE) pH 9.0 followed by general‐protein staining of gels. A major postalbumin of fox plasma was identified as α 1 B‐glycoprotein (α 1 B) by using immunoblotting with antiser m specific to human or pig plasma α 1 B. Four codominant, autosomal alleles of α 1 B were found in arctic foxes. Two transferrin (TF) alleles (Tf F , Tf S ) were observed in arctic foxes and two (Tf D , Tf F )in silver foxes; the TF F type of both of the fox species showed identical electrophoretic mobilities. The arctic foxes showed a high degree of polymorphism for both TF and α 1 B. The silver foxes showed a scarce polymorphism of TF and were monomorphic for α 1 B. The arctic fox, silver fox and their hybrids could be clearly differentiated from one another by their plasma protein patterns obtained by the PAGE method.