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Identification of bovine K‐casein genotypes at the DNA level
Author(s) -
RANDO A.,
GREGORIO P. DI,
MASINA P.
Publication year - 1988
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1988.tb00789.x
Subject(s) - kappa , psti , biology , restriction enzyme , microbiology and biotechnology , genotype , complementary dna , ecori , endonuclease , dna , genetics , restriction fragment length polymorphism , gene , philosophy , linguistics
Summary. By using a bovine K‐Cn cDNA as probe and the Pst I endonuclease we demonstrate that the DNA restriction patterns of K‐ Cn AA and K‐ Cn BB cows are different. Besides two invariant fragments (about 6.8kb and 1.1 kb) the former shows two fragments of about 4.3 kb and 0.3 kb and the latter one fragment of about 4.6kb. K‐ Cn AB cows show intermediate pattern. Therefore, it is possible to determine the bovine K‐ Cn genotypes even in absence of gene product.