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Analysis of a horse family with a crossing‐over between the ELA complex and the A blood group system
Author(s) -
GUERIN G.,
VAREWYCK H.,
BERTAUD M.,
CHASSET P.
Publication year - 1988
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1988.tb00782.x
Subject(s) - biology , genetics , restriction fragment length polymorphism , locus (genetics) , southern blot , complementary dna , haplotype , gene , microbiology and biotechnology , horse , polymerase chain reaction , allele , paleontology
Summary. A horse family in which a recombination occurred in the chromosome region coding for the serological specificities of the ELA complex and those of the A blood group system of a mare was further analysed by mixed lymphocyte reaction (MLR) and Southern blot hybridization. This family consisted of a stallion, a mare and five full sibs. The stallion and the mare were heterozygous for internationally recognized ELA specificities while only the mare was heterozygous for the A blood group system. MLR between all members of the family confirmed that the stallion possessed two different ELA haplotypes and suggested that recombination in the mare occurred outside the segment delimited by the ELA‐A locus and the MLR region. DNA samples from all individuals were investigated by Southern blot analysis using three restriction enzymes (Eco RI, Hind III or Taq I), three human HLA probes (one of class I cDNA and two of class II probes), one cDNA (DRß) and one genomic (DQα). Class I and class II restriction fragments of the mare segregated in accordance to the ELA specificities and thus clearly confirming that the crossing‐over did not occur between the ELA‐A gene and the class I, class II region nor between DRß and DQα subsets. The A blood group genetic determinants would thus be situated outside the ELA region defined by class I and class II genes.