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Production of alloantibodies against caprine lymphocyte antigens
Author(s) -
NESSE L. L.,
LARSEN H. J.
Publication year - 1987
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1987.tb00755.x
Subject(s) - biology , histocompatibility , typing , immunology , peripheral blood mononuclear cell , tissue typing , antigen , major histocompatibility complex , andrology , lymphocyte , whole blood , human leukocyte antigen , medicine , genetics , in vitro
Summary. In all, 217 primiparous goats were each injected with blood cells from their own newborn kid. Eighty‐five goats were given mononuclear cells, 61 were given leucocytes and 71 received whole blood. The goats were injected one, two or three times before collection of sera. Sera were also collected from 42 non‐injected, primiparous goats. The sera were compared with regard to their potential value in class I histocompatibility typing. The percentage of potentially valuable sera was highest in the group of animals injected twice with whole blood (66.7%). However, this percentage was not significantly higher than the percentage in the group of animals injected once with whole blood (54.7%). It is concluded that injecting primiparous goats once with whole blood from their own newborn kid, is a rapid and easy method, which gives a high yield of alloantisera with potential value in class I histocompatibility typing.