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Polymorphic restriction sites in the horse (β‐globin gene cluster
Author(s) -
RANDO A.,
GREGORIO P.,
MASINA P.
Publication year - 1986
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1986.tb03196.x
Subject(s) - biology , genetics , allele , restriction fragment length polymorphism , microbiology and biotechnology , restriction fragment , bamhi , gene , gene cluster , globin , psti , phenotype , restriction site , genotype , plasmid , restriction enzyme
Summary Horse DNA samples digested with PstI and probed with the rabbit β 1 globin gene show three phenotypes determined by one fragment of variable length (about 5·1 or 3·3 kb). Family data demonstrate that these fragments segregate as Mendelian alleles. The frequencies of the two alleles are 0·66 for the 3·3‐kb fragment and 0·34 for the 5·1‐kb one. Another polymorphism has been detected with BamHI. Again three phenotypes determined by two alleles (fragments of 7·5 and 3·8 kb) have been observed. Allelic frequencies of the 7·5‐ and 3·8‐kb fragments are 0·24 and 0·76 respectively. The two polymorphic sites are non‐randomly associated.