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Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls *
Author(s) -
BECKMANN J. S.,
KASHI Y.,
HALLERMAN E. M.,
NAVE A.,
SOLLER M.
Publication year - 1986
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1986.tb03185.x
Subject(s) - biology , restriction enzyme , restriction fragment length polymorphism , genetics , locus (genetics) , haeiii , terminal restriction fragment length polymorphism , allele , restriction fragment , polymorphism (computer science) , population , genotype , microbiology and biotechnology , dna , gene , demography , sociology
Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations.