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Transferrin variants in sheep: separation and characterization by polyacrylamide gel electrophoresis and isoelectric focusing*
Author(s) -
ERHARDT G.
Publication year - 1986
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1986.tb00727.x
Subject(s) - isoelectric focusing , transferrin , polyacrylamide gel electrophoresis , gel electrophoresis , biology , chromatography , electrophoresis , biochemistry , microbiology and biotechnology , chemistry , enzyme
Summary. Isoelectric focusing with carrier ampholytes in ultrathin polyacrylamide gels and polyacrylamide gel electrophoresis in a discontinuous buffer system were used for the separation of sheep transferrin variants. For identification of the different iron‐binding sites of transferrin a stepwise urea gradient, different degrees of iron saturation and double one‐dimensional electrophoresis were used. Isoelectric focusing results in an increased resolution of the Fe 0 ‐transferrin, Fe 1 ‐transferrin and Fe 2 ‐transferrin region. At the level of Fe 0 ‐transferrin and Fe 1 ‐transferrin the variants I, A, G, B, C, D, M, E, Q, P can be identified. The method is especially suitable for genetic studies. For screening purposes up to 108 samples can be separated within one run in an ultrathin gel.