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Genomic hybridization of bovine class II major histocompatibility genes: 2. Polymorphism of DR genes and linkage disequilibrium in the DQ‐DR region
Author(s) -
ANDERSSON L.,
BóHME J.,
PETERSON P. A.,
RASK L.
Publication year - 1986
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1986.tb00723.x
Subject(s) - biology , genetics , haplotype , restriction fragment length polymorphism , linkage disequilibrium , gene , major histocompatibility complex , genetic linkage , restriction enzyme , southern blot , allele , genotype
Summary. Class II genes of the bovine major histocompatibility complex have been investigated by Southern blot analysis using human cDNA probes for DQα, DQβ, DRα and DRβ. In this report restriction fragment length polymorphisms of DR α and DR β are described. The polymorphisms were interpreted genetically by analysing five paternal half‐sib families of the Swedish Red and White Breed, comprising altogether 28 offspring. Using the restriction enzymes Bam HI, Eco RI and Pvu II, three DRα and three DRβ allelic fragment patterns were resolved. The DR α and DR β genes thus appear to be much less polymorphic than the previously described DQ α and DQ β genes. Also, the observed linkage disequilibrium between DR genes was less pronounced than that between DQ genes, whereas the association between DR and DQ haplotypes was very strong. The family data available indicated strongly that the DQ α, DQ β, DR α and DR β genes are all closely linked.