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Haemopexin in sheep, mouflon and goat: genetic polymorphism, heterogeneity and partial characterization *
Author(s) -
Stratil A.,
Glasnák V.,
Tomášek V.,
Williams J.,
Clamp J.
Publication year - 1984
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1984.tb01128.x
Subject(s) - biology , sialic acid , allele , neuraminidase , immunoelectrophoresis , phenotype , biochemistry , haptoglobin , orosomucoid , glycoprotein , amino acid , genetics , microbiology and biotechnology , gene , endocrinology , antigen , enzyme
Summary Benzidine staining of starch gels after electrophoresis of sera to which haematin was added revealed polymorphism of haemopexin in sheep, mouflon and goat. In sheep three phenotypes were observed, Hpx A, Hpx AB and Hpx B. Pedigree data support the hypothesis of codominant inheritance from a single locus by two alleles, Hpx A and Hpx B . Neuraminidase treatment of haemopexin preparations showed that Hpx B covered two variants, B1 and B2, thus indicating genetic control by three alleles ( Hpx A , Hpx B1 and Hpx B2 ). In sheep populations the frequency of Hpx B is low. In mouflon, in addition to the two variants that are like those of sheep, absence of haemopexin was observed in some animals, by using starch gel electrophoresis as well as immunoelectrophoresis. In goat, three phenotypes were detected, Hpx A, Hpx AB and Hpx B, differing in migration from those of sheep. Haemopexins of the studied species are heterogeneous. Sialic acid is responsible for electrophoretic heterogeneity of sheep haemopexin. Chemical. composition (amino acid and carbohydrate), molecular weight (56 060) and N‐terminal sequence (Leu‐Pro‐Pro‐) of sheep haemopexin were also determined.