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The transfer of bovine J blood group activity to erythrocytes: chemical nature of transferable and of non‐transferable J 1
Author(s) -
Wagner R.,
Wrenger M.,
Oulevey J.,
Thiele O.
Publication year - 1984
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1984.tb01126.x
Subject(s) - pronase , glycosphingolipid , ceramide , glycoprotein , biochemistry , globotriaosylceramide , chemistry , chromatography , membrane , red blood cell , erythrocyte membrane , enzyme , trypsin , fabry disease , medicine , apoptosis , disease , pathology
Summary The bovine J blood group substance exists as a glycosphingolipid (ceramide deca‐hexoside as well as ceramide dodecahexoside) and as a glycoprotein. The lipidic form occurs in erythrocyte membranes, both forms are found in serum. The lipidic J substances were isolated from erythrocytes and from serum, and identified by thin‐layer chromatography with lipidic J substances isolated from spleen. The glycoprotein nature of the non‐lipidic J of serum was evident by pronase‐catalysed hydrolysis yielding J‐active glycopeptides of lower molecular weights. The lipidic J was completely extracted from lyophilized stroma with chloroform/methanol. From lyophilized serum, however. it was completely extracted only in the presence of water, indicating different binding partners in serum and in erythrocyte membranes. The J lipid was incorporated as intact molecule into the erythrocyte membrane by a simple incubation technique. The incorporation was inhibited by various glyc‐erophospholipids (called blockers). The J glycoprotein could not be transferred to the erythrocyte membrane. Three methods are descrjbed which are suitable for the preparation of a blocker‐free fraction enriched with J lipids from J‐positive serum.