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A method for freezing bovine lymphocytes
Author(s) -
Oliver R. A.,
Springbett A. J.,
Spooner R. L.
Publication year - 1984
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1984.tb01118.x
Subject(s) - cryoprotectant , biology , chromatography , chemistry , cryopreservation , genetics , embryo
Summary A simple two‐stage technique for preserving bovine lymphocytes is described. Lymphocytes from animals chosen at random were used. The experiments indicate that the optimum temperature for freezing and the optimum concentration of dime‐thylsulphoxide (DMSO) as cryoprotectant were in the range ‐29 °C to ‐31 °C and 17.5 % to 20 % respectively. These concentrations of DMSO are much greater than those reported in most other studies.