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Serological and genetic identification of a bovine B lymphocyte alloantigen system
Author(s) -
Newman M. J.,
Adams T. E.,
Brandon M. R.
Publication year - 1982
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1982.tb01051.x
Subject(s) - antiserum , antigen , biology , buffy coat , antibody , serology , lymphocyte , immunology , microbiology and biotechnology , cytotoxic t cell , virology , genetics , in vitro
Summary A two‐colour fluorescence micro cytotoxicity test was used to screen antisera for antibodies specific for bovine B lymphocytes. A total of 114 cattle alloantisera were screened against peripheral blood lymphocytes from 100 unrelated individuals. Anti‐B lymphocyte activity was detected in 47 antisera. Cytotoxic antibodies to antigens other than B lymphocyte specific antigens were removed by absorbing the antisera with buffy coat cells or platelets isolated from whole blood. Selected antisera were used to type paternal half‐sib families. The presence of a polymorphic, MHS‐linked antigen system on B lymphocytes was demonstrated. The tissue distribution and MHS linkage of these antigens suggests this system is analogous to the class II or Ia antigens of other species.