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Alcohol dehydrogenase isozymes in the mouse: Genetic regulation, allelic variation among inbred strains and sex differences of liver and kidney A 2 isozyme activity
Author(s) -
Holmes Roger S.,
Duley John A.,
Imai S.
Publication year - 1982
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1982.tb01048.x
Subject(s) - isozyme , biology , genetics , allele , alcohol dehydrogenase , inbred strain , genetic variation , gene , enzyme , biochemistry
Summary Genetic analysis of a proposed cis‐acting temporal locus ( Adh‐3t ), which regulates alcohol dehydrogenase C 2 (ADH‐C 2 ) acitivity in mouse epididymis extracts, among F 1 (ddN × BALB/c) × ddN male backcross progeny provided evidence for genetic distinctness between the structural ( Adh‐3 ) and temporal ( Adh‐3t ) loci on chromosome 3. Genetic analysis also confirmed the close, linkage of Adh‐1 (encoding liver and kidney ADH‐A 2 ) and Adh‐3 (encoding stomach ADH‐C 2 ) to within 0.3 centimorgans on the mouse genome. Evidence is presented for a proposed closely linked cis‐acting temporal locus (designated Adh‐1t ) for the A 2 isozyme (encoded by Adh‐1 ) controlling the activity of this enzyme in mouse kidney extracts, but having no apparent affect on liver and intestine ADH‐A 2 activities. An extensive survey of the distribution of Adh‐1, Adh‐3 and Adh‐3t alleles among 65 strains of mice is reported — with the exception of two Japanese strains (ddN and KF), linkage disequilibrium between Adh‐3 and Adh‐3t was observed. Sex differences in mouse liver and kidney ADH‐A 2 activities were observed, with male/female ratios of approximately 0.6 and 3 respectively for these tissue extracts.