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A polymorphic antigen displaying different molecular weights in sheep and cattle sera
Author(s) -
Benedictis Giovanna
Publication year - 1981
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1981.tb01548.x
Subject(s) - protein subunit , antigen , biology , polyacrylamide gel electrophoresis , molecular mass , size exclusion chromatography , microbiology and biotechnology , gel electrophoresis , biochemistry , genetics , enzyme , gene
Summary The polymorphic antigen Bl is present, exhibiting different molecular weights, in both sheep and cattle sera. The molecular weight of the antigen, purified from both sera by a specific immunoabsorbent, was analysed by SDS polyacrylamide gel electrophoresis and calibrated gel filtration. Results indicate that only one subunit, 35 000 MW, forms cattle Bl while a further subunit, 60 000 MW, is present in sheep Bl. The antigen activity is localized on the 35 000 MW subunit, which appears to be the same in both species.