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Characterization of class II histocompatibility antigens in pigs
Author(s) -
Chardon Patrick,
Renard Christine,
Vaiman Marcel
Publication year - 1981
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1981.tb01532.x
Subject(s) - immunoprecipitation , biology , antigen , polyacrylamide gel electrophoresis , major histocompatibility complex , population , microbiology and biotechnology , gel electrophoresis , molecular mass , lectin , molecule , biochemistry , chemistry , genetics , gene , enzyme , demography , organic chemistry , sociology
Summary The D region of the SLA complex in the pig has been studied by immunochemical and sequential immunoprecipitation techniques as well as the redistribution of membranous antigens (capping). The molecules identified by the anti‐la sera were solubilized by NP 40, purified on lectin and precipitated. Polyacrylamide gel electrophoresis under dissociating conditions shows that these molecules are made up of two chains whose molecular weights are 32 000 and 26 000 daltons respectively. Sequential immunoprecipitation and capping experiments indicate that two distinct types of la molecules exist. At least a part of the nylon‐wood‐adherent lymphocyte population expresses both types of molecules.